Fig. 5. The wnt8^– expanded organizer phenotype is due to reduced vent and vox expression. (A) Rescue of wnt8 mutants by Vox. gsc expression (bracket) in wild-type (a,c) or wnt8^– (b,d) embryos, uninjected (a,b) or injected (c,d) with a vox expression plasmid. Some isolated lateral cells still express gsc in injected wnt8^– embryos because of the mosaic expression of Vox (panel d, arrow). Embryos shown are at 70% epiboly, dorsal view. (B) Reduction of Vent/Vox enhances the wnt8^– organizer phenotype. Graph shows the percentage of embryos belonging to a specific phenotypic class. Class I, wild-type chd expression; class II to IV, increasingly expanded chd expression. 100% of wild-type and wnt8^– embryos belong to class I and class II, respectively. Upon injection of vent+vox MOs, most wild-type embryos belong to class II (96.5%, n=85), whereas wnt8^– embryos belong to both classes III and IV (60.7% and 39.3%, respectively, n=28). Embryos shown at the bottom of the graph are at shield stage, animal view, dorsal right.