Fig. 1. Altered expression of Raldh3 detected by immunohistochemistry (IHC) in Gsh2 mutants. (A) At E12.5, Raldh3 is normally expressed in the SVZ of the ventromedial LGE. (B) In Gsh2 mutants, the Raldh3 staining is reduced and its normal expression domain in the LGE is truncated (arrow). However, the expression of Raldh3 in the nasal epithelium (NE) and retina (R) remains at wild-type levels. (C) At E16.5, Raldh3 is expressed in the SVZ of the LGE and septum, and (D) remains severely reduced in the Gsh2 mutant LGE. NE, nasal epithelium; R, retina; Stm, striatum.