Fig. 2. The induction of Shh and its targets at the ventral forebrain is
delayed or attenuated at E8.5. Whole-mount in-situ hybridization of
Shh (A-D); Nkx2.1(E-H); Nkx2.2 (I-L);
Ptch1 (M-P) of E8.5 dpc wild-type and mutant embryos as indicated.
Arrow in (A) indicates the Shh expression in the axial mesoderm
notochord. Arrowhead in (B) indicates expression in the ventral forebrain.