Fig. 3. Expression patterns of PTL assessed using RT-PCR. All tissues were from the Columbia ecotype except lane 11 (Ler). The same total RNA samples were used for three reactions in each case, using mRNA specific primers for either PTL (top), flower-specific APETALA3 amplified using essentially the same conditions (centre), or ACTIN2 as a control (bottom). 3.5 µl of the reaction was loaded for PTL and AP3 reactions, 1.5 µl for the ACT2 control. In lane 6, the receptacle at the base of the siliques was removed before RNA extraction.