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Fig. 1. Analysis of Phox2b regulatory regions in transgenic mice. (A) The Phox2b locus and lacZ reporter constructs used in transgenic mouse assay. Black boxes represent Phox2b exons I, II, and III; the red box represents the ß-globin (ß-glo) minimum promoter. (B,D) Dorsal (B) and lateral (D) views of E10.5 embryos carrying the P2b_10/lacZ construct. lacZ expression is detected by whole-mount in situ hybridization. (C,E) Dorsal (C) and lateral (E) views of E10.5 embryos carrying the P2b_2.8/lacZ construct, stained for ß-gal activity. Expression of P2b_10/lacZ recapitulates most of Phox2b expression pattern in hindbrain and sensory ganglia (Pattyn et al., 1997) (B,D), whereas that of the P2b_2.8/lacZ is selectively restricted to ventral r4 (C) and not expressed in the ganglia (E). r, rhombomere; nIII, oculomotor nucleus; nIV, trochlear nucleus; lc, locus coeruleus; gVII, gIX and gX, geniculate, petrose and nodose ganglia of cranial nerves, respectively.