Fig. 2. mRNA is affected in mutant embryos. (A) RT-PCR on cDNA from mutant and wild-type hi3308 embryos with a pair of hi3308/seahorse specific primers flanking the insertion site. (B) Control RT-PCR with a pair of actin specific primers. A serial dilution of cDNA was used to show that similar amounts of cDNA were used. m, 1 kb ladder; wt, wild type; mu, mutant; 1-4, 1:10, 1:200, 1:4,000 and 1:80,000 dilutions.