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Fig. 4. Ciliary defects in scorpionhi459. (A) The pronephric duct region (arrow) in a wild-type embryo at 50 hpf reacts strongly with an antibody against acetylated tubulin. (B) Confocal image shows cilia stained with this antibody in the pronephric duct of a wild-type embryo at 24 hpf. (D) Defect of the cilia in scorpionhi459 at 50 hpf; (D) enlarged view of C. wt, wild type; 459, scorpionhi459.