Fig. 5. Vascular defects in Pod1 KO gonads. Migration assays with GFP-expressing (green) wild-type mesonephros, and wild-type (A,C,E) or mutant (B,D,F) gonads at 72 hours, demonstrated a marked decrease in migration of endothelial cells (red) into Pod1 KO gonads. Yellow denotes double-staining of GFP and CD31/PECAM-positive cells, confirming the presence of mesonephric-derived vascular networks in wild-type but not Pod1 KO gonads. At 13.5 dpc, pericytes (per) were observed in close association with endothelial (en) cells of wild-type gonadal capillaries (G,I), but were never seen around Pod1 KO capillaries (H,J).