Fig. 7. Early female-specific markers are expressed in Pod1 KO XX gonads. Whole-mount in situ hybridization for Wnt4 (A) and follistatin (B) is shown in XY and XX gonads from 12.5 dpc embryos. Expression of Dmc1, a marker of meiotic germ cells (C), is shown in 14.5 dpc embryos. Wnt4 is expressed in control XX gonads and mesonephroi, and was increased in mesonephroi of XX mutants (–/–). Follistatin is also expressed in XX mutant gonads, although the expression is somewhat decreased as compared with controls. Dmc1 expression is similar in XX mutants and controls.