Fig. 4. Coincidence of nerve and muscle fibers in T. rude. A view of five segments (95-105 hours clonal age) in a germinal plate immunostained with anti-ACT (green) and Lan3-14 (red) to identify axons and muscle fibers, respectively. The right N teloblast was labeled with RDA (blue). More than 30 bilaterally paired, longitudinal muscle fibers are apparent; between 20-25 circular muscle fibers can be identified per segment. Arrows indicate the largest gap between identifiable circular muscle fibers and correspond to the position of the iterated MA nerve tract. Other nerve tracts (AA, PP and UP) consistently align between characteristic sets of muscle fibers. Anterior is upwards. Scale bar: 25 µm.