Fig. 2. Bmp7 is required for normal development of the lacrimal gland. All preparations are stained with X-gal. (A) E13.5 P6 5.0-lacZ mouse and (B) Bmp7^m1Rob/m1Rob, P6 5.0-lacZ mouse both showing normal formation of the primary lacrimal bud. (C) Wild-type E19.5 P6 5.0-lacZ mouse showing normal lacrimal gland branching pattern. The gland is composed of a small intra-orbital lobe (io) (blue arrowhead) and a large exorbital lobe (xo). (D-H) Lacrimal glands in E19.5 Bmp7^m1Rob/m1Rob, P6 5.0-lacZ mice showing the position of the intraorbital lobe (blue arrowheads), misplaced buds and branches (purple arrowheads) and in some cases (E,F) loss of lobe distinctions. The degree to which the lacrimal gland was affected did not correlate with the degree of micropthalmia (mi). (H) Lacrimal gland dissected from E19.5 Bmp7^m1Rob/m1Rob, P6 5.0-lacZ mouse (black arrowheads indicate the extent of gland mesenchyme).