Fig. 3. Localization and expression of transgenic GFP-fusion proteins. For each transgenic genotype, localization of GFP is demonstrated in early and late stages of oogenesis: (A,B) vas⁺; (D,E) vas^616-618; (G,H) vas⁶¹⁶; (J,K) vas⁶¹⁷; (M,N) vas⁶¹⁸. Protein expression in ovaries of transgenic flies is demonstrated on western blots probed with anti-Vas. (C) Vas+; (F) Vas616-618; (I) Vas616; (L) Vas617; (O) Vas618. Flies contained two copies of each transgene in the wild-type background. For each sample, the faster migrating band represents endogenous Vas and the slower migrating band represents the transgenic GFP-Vas.