Fig. 6. Pole plasm assembly in the presence of reduced Vas-eIF5B interaction. (A) Western blot of ovarian extracts from wild-type (OreR), vas^PD, vas^PD;P{vas⁺} and vas^PD;P{vas⁶¹⁷} females was probed with anti-Vas. The same blot was probed with anti--Tubulin as a loading control. (B-M) Stage 10 ovaries from OreR, vas^PD, vas^PD;P{vas⁺} and vas^PD;P{vas⁶¹⁷} females were labeled for Osk (B-E), Vas (F-I) and Tud (J-M) by immunostaining.