Fig. 5. (A-C) At stage 17, in the DD2-misexpressed limb, Meis2 was induced (B) in an area where co-expressed EGFP proteins (shown in green) were detected (A). Normal expression was shown in C. (D-F) At stage 19, similar but more extensive induction of Meis2 was observed. (G,H) Implantation of BMP4-soaked beads repressed Meis2 expression (red arrowheads) in both a proximal area (G) and a distal end (H), with a deformity of the AER (H). (I) Misexpression of DD2 cancelled the BMP4-mediated repression of Meis2, with expansion of Meis2 expression to the distal end. (J) A luciferase reporter plasmid containing 700 bp 5' region of human MEIS1 was transfected into HepG2 cells, along with BMP4, Smad1, Dach1, VP=Dach1 and mouse Sin3a expression plasmids. Expression of BMP4, Smad1 and Dach1 repressed this reporter. This repression was enhanced by the addition of mouse Sin3a. By contrast, when VP=Dach1 was expressed, activation of this reporter was obtained.