Fig. 5. Decreased number of GABAergic interneurons in the cortex of Vax1 mutant mice. GABA-positive cells were counted in sagittal sections from wild-type (A,C) and mutant (B,D) P0 mice. (A,B) DAPI staining of two comparable sections from wild-type (A) and mutant (B) brains. (C,D) Immunohistochemistry with FITC-labeled anti-GABA antibody on the same sections; images are high magnifications of the regions indicated with asterisks in A and B, respectively. (E) Summary of cell counts (see text for details). Graphical representation of the results, indicating the amount of GABAergic cell decrease in Vax1-knockout brains (wild type is 100%). Scale bars: 500 µm in A,B; 50 µm in C,D.