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Fig. 1. Phylogenetic relationships of dipteran eve, h and wg genes. Newly-identified dipteran eve (A), h (B) and wg (C) sequences are more closely related to one another than to paralogous Drosophila genes. Phylogenetic distance trees were generated from ClustalW alignments of predicted protein sequences (see legend to Fig. S1 at http://dev.biologists.org/supplemental) using the Quartet Maximum-Likelihood Method of Strimmer and von Haeseler (Strimmer and von Haeseler, 1996). Numbers refer to reliability values in percent. AbdominalA (Dme-AbdA), AbdominalB (Dme-AbdB), Deadpan (Dme-Dpn), E(spl)m{Delta} (Dme-HLHm{Delta}), Hairy/E(spl)-related with YRPW motif (Dme-Hey), Ultrabithorax (Dme-Ubx), Dme-Wnt2, Dme-Wnt5 and Dme-Wnt6 were used as outgroups. Aga, Anopheles gambiae; Cal, Clogmia albipunctata; Cfu, Coboldia fuscipes; Dme, Drosophila melanogaster; Eba, Episyrphus balteatus; Eli, Empis livida; Hpl, Haematopota pluvialis; Mab, Megaselia abdita; Pco, Platypeza consobrina. Accession numbers: Dme-AbdA SWP, P29555; Dme-AbdB SWP, P09087; Dme-Dpn SWP, Q26263; Dme-HLHm{Delta} SWP, Q01071; Dme-Eve SWP, P06602; Dme-Hairy SPTREMBL, Q95NU9; Dme-Hey SPTREMBL, Q9U9U4; Dme-Ubx SWP, P02834; Dme-Wg SPTREMBL, Q8MQP9; Dme-Wnt2 SPTREMBL, Q9V584; Dme-Wnt5 SPTREMBL, Q9VWT4; Dme-Wnt6 SPTREMBL, Q9VM26.