Fig. 1. XNIF contains the conserved NIF motif. (A) Nucleotide and predicted amino acid sequence of XNIF (accession number: AY280863). The start codon is marked by bold letters. Upstream stop codons of the same reading frame are marked by boxes. The stop codon is marked by an asterisk. A fragment that directs vegetal localization in stage I and later stage oocytes is underlined (nt 252-551). The conserved NIF-motif of XNIF is highlighted in the amino acid sequence. The conserved phosphatase motif DXDXT/V is boxed. (B) Amino acid sequence comparison of NIF-domains of XNIF and related (hypothetical) proteins from chicken (NIF isoform T1; AF189773), mouse (NIF-like protein; AJ344340), human (Hya22; NM_005808) and two Xenopus proteins (XOs4, AF441288, and Dullard, AB084264). Hyphens indicate amino acid positions identical to XNIF sequence; dots reflect deletions. The conserved phosphatase motif DXDXT/V is boxed.