Fig. 5. Effects of Ras^S17N misexpression on Wnt1, Pax2, Pax5, En1, En2 and Fgf8. Effects on Wnt1 (A,B), Pax2 (C-G), Pax5 (H-L), En1 (M-Q), En2 (R-V). Brown, immunohistochemical staining against HA-tag. Blue, signal for in-situ hybridization. Dorsal view (A,B), Left (control) side of the brain vesicles (C,H,M,R), right (transfected) side (D,E,I,J,N,O,S,T). Higher magnification (F,G,K,L,P,Q,U,V). The areas enclosed by the dashed line on (E,J,O,T) corresponds to (G,L,Q,V), respectively. Wnt1 was induced in the dorsal metencephalon (arrowheads on A). Expression of Pax2/5 and En1/2 were repressed by Ras^S17N misexpression. Scale bars: 200 µm.