Fig. 2. Expression of the five predicted miR164 targets and of CUC3 in 2x35S::miR164 lines. (A) RT-PCR analysis of CUC1, CUC2 and CUC3 mRNA accumulation in inflorescence apices of wild-type plants, 2x35S::erGFP and representative 2x35S::miR164A and 2x35S::miR164B lines showing either a weak (W), intermediate (I) or strong (S) flower phenotype. APT was used as a control. (B) RT-PCR analysis of At5g61430, At5g07680 and NAC1 mRNA accumulation in inflorescence apices. Histograms show quantification of the target expression level relative to the APT control.