Development -- Marklund et al. 131 (17): 4323 Figure IG5

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Fig. 5. Retinoic acid (RA) induces intermediate character in early ventral telencephalic cells. (A) A stage 8 chick embryo (dorsal view, rostral is upwards). Grey square indicates ventral telencephalic neuroectoderm explant region. (B) Expression of transcription factors in stage 8 ventral (V, st 8) explants cultured alone or with all-trans retinoic acid for 48-52 hours. Stage 8 V explants (n=15) generated NKX2.1⁺ cells (>90%) and ISL1⁺ cells (19±4%) but no MEIS2⁺ or PAX6⁺ cells. Stage 8 V explants (n=15) exposed to all-trans retinoic acid generated MEIS2⁺ cells (23±3%), ISL1⁺ cells (17±4%), PAX6⁺ cells (15±4%) but no NKX2.1⁺ or EMX1⁺ cells. Scale bar: 30 µm.