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Fig. 3. tra-1 and ehn-3 redundantly control SGP development. (A,B) Young adults, DAPI stained; male tail and pharynx are indicated. (A) tra-1(e1099) single mutant with a normally shaped male gonad (bracket). (B) tra-1(e1099); ehn-3(q766) double mutant with no visible gonad (arrow). (C,D) L4/young adults, stained with antibodies to germ cell marker PGL-1. (C) ehn-3(q766) single mutant with normal two-armed gonad (brackets). Actual genotype is tra-1/+; ehn-3. (D) tra-1(e1099); ehn-3(q766) double mutant with only a few germ cells. (EH) L1 larvae expressing pes-1::GFP, an SGP-marker (Molin et al., 2000). (E,G) DIC; (F,H) immunofluorescence of same animal shown in E and G, respectively. (E,F) tra-1(e1099) mutant L1. (E) SGP (Z4, white arrow) has typical morphology. (F) Z4 expresses pes-1::GFP; (G,H) tra-1(e1099); ehn-3(q766) double mutant L1. (G) A small cell is associated with PGCs (white arrow); (H) this small cell does not express pes-1::GFP.