Fig. 4. Gonadal masculinization in tra-1/+; ehn-3. (A-D) Gonadal leader cells marked with lag-2::GFP (Blelloch et al., 1999). (A) Wild-type XX hermaphrodite leader cell (DTC) is crescent shaped. (B) Wild-type XO male leader cell (LC) is round. (C,D) tra-1/+; ehn-3(q689) XX leader cells. (C) Leader cell with typical DTC crescent shape (most common). (D) Leader cell with typical LC round shape. A similar low-penetrance gonadal masculinization was also observed in tra-1/+; ehn-3(RNAi). (E,F) tra-1/+; ehn-3(q689) XX larvae, DIC. (E) Gonadal arm with normal hermaphrodite organization: DTC (white arrow) is adjacent to germ cells (bracket) and separated from other somatic gonadal cells (out of photo). (F) Gonadal arm with male organization: LC (white arrow) is adjacent to somatic gonadal cells (black arrows) and separated from germ cells.