(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Arabidopsis PIR and human SRA1 have interchangeable in vivo functions and interactions with ATNAP125. (A) Direct interaction of recombinant PIR and ATNAP125 in a GST-pull-down assay. (Top) Lanes 1-5, fractions probed with anti-ATNAP antibody; (bottom) same fractions probed with control antibody anti-AT3G. Lane 1, 5% of total binding reaction; lane 2, GST-PIR bead bound pellet fraction; lane 3, GST-PIR bead unbound; lane 4, GST bead bound; lane 5, GST bead unbound. (B)PIR and human SRA1 interact with ATNAP125 in a yeast two-hybrid assay. The bait and prey plasmids that were co-transformed into yeast are defined to the left of the corresponding yeast patches and ß-galactosidase assay results. pDS32PIR, full-length PIR; pDS32HSPIR, full-length human SRA1; pDS22ATNAP, full-length ATNAP125; pDS22HSNAP, full-length human NAP125. (C) Overexpression of human SRA1 can suppress pir-3 trichome distortion. Left to right: Col mature trichome; pir-3 mature trichome; mature trichome on a Col plant that harbors a human SRA1 overexpression construct; mature trichome on a pir-3 plant that harbors a human SRA1 overexpression construct. Scale bars: 100 µm.