Fig. 6. Localization and quantification of actin filaments in whole mounted wild-type (Col), dis2 and pir stage 4 trichomes. (A-C) Wild-type. (D-F) dis2-1. (G-I) pir-3. (A,D,G) Maximum projection of whole-cell F-actin signals in stage 4 trichomes. (B,E,H) Maximum projection of signals from cortical actin filaments (within 2.5 µm of the plasma membrane) in the same branches of A, D and G, respectively. (C,F,I) Maximum projection of signals from actin filaments in the core branch cytoplasm. (J) Mean ratios±s.d. of core cytoplasmic to total cytoplasmic actin filament signals in stage 3/4 and stage 4/5 branches from 5-10 branches. Broken line indicates the lower limit of the 95% confidence level of the mean ratio for wild-type stage 4/5 trichomes. Scale bars: 10 µm.