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Fig. 7. Interaction of the PHD domain of chick Pcl2 with EZH2. 293T cells were transfected with expression vectors expressing the FLAG-tagged chick Pcl2, the FLAG-tagged PHD fingers (AA103-238) and the Myc-tagged mouse EZH2. Cell extracts were immunoprecipitated and blotted with antibodies against FLAG or Myc, reciprocally. Lane 1, negative control; lane 2, positive control for Myc pull-down and blot (arrow indicates the blotted band); lanes 3 and 4, positive control for FLAG pull-down and blot; lane 5, pull-down and blot of chick Pcl2-FLAG and EZH2-Myc reciprocally; lane 6, pull-down and blot of PHD-FLAG and EZH2-Myc reciprocally. Data shown are representatives of at least three independent experiments.