Fig. 3. E4BP4 enhances cell body growth. (A) Histograms for cell body area from a single experiment. Motoneurons electroporated with GFP were cultured without trophic factors (control), or with neurotrophic factor (BDNF). Motoneurons expressing E4BP4 were cultured without BDNF. Cell body area for 80-200 neurons in each condition was measured 1 day later and expressed in arbitrary pixel units. There is a clear shift to larger sizes in motoneurons expressing E4BP4. (B) Median cell body areas in each condition (mean±s.e.m.; n=5 independent experiments). Statistical significance was calculated by Student's t-test (two-tailed). The addition of BDNF to E4BP4 electroporated motoneurons did not further increase cell body size (7.3±0.3 pixels; n=3).