Fig. 4. E4BP4 potently stimulates axonal outgrowth. (A) Typical images of control and E4BP4-expressing motoneurons after 1 day in culture, as used for subsequent image analysis. Scale bar: 20 µm. (B) Cumulative plot of length of longest neurite from one representative experiment. For each value of neurite length, the percentage of the total number of neurons having a neurite at least that length is plotted. A shift of the curve to the right thus indicates increased axonal growth. Values from control neurons (black line), control neurons with BDNF (broken line) and E4BP4-expressing neurons without BDNF (grey line) are shown. (C) Median values for length of the longest neurite, expressed relative to control neurons with BDNF, fixed as 1 (mean±s.e.m.; n=5 independent experiments).