Fig. 5. E4BP4 is not regulated by survival signalling pathways in motoneurons. Semi-quantitative RT-PCR and western-blot analysis of E4BP4 expression in cultured motoneurons in the presence or absence of trophic support 6 hours after plating. (A) Radioactive Southern-blot analysis of RT-PCR amplified transcripts. (B) Western blots showing E4BP4 and -tubulin expression. (C) Relative band intensities of RT-PCR and Western-blot analysis. Each band was scanned and its intensity analysed by Image J software. Each single E4BP4 band was normalised with respect to its corresponding actin or tubulin control band and then the values for parallel cultures with or without trophic factors were expressed as an intensity ratio (see Materials and methods). Mean values are plotted (mean±s.e.m.; n=30 independent RT-PCR experiments and n=10 western blots).