Fig. 3. ATH5-expressing cells in the developing chick retina. (A,B) Serial transverse sections at stage E2.5 were hybridized with an ATH5-specific antisense riboprobe. ATH5 is expressed in the central retina (gray) in a domain corresponding to 30% of the surface of the retina. (C) Cell counting reveals that 88% of the cells in the central domain express ATH5, representing 26% of the total retinal cell population. The dissociated cells of E6 retinas were hybridized with an ATH5-specific antisense riboprobe and the ration of labeled to unlabeled cells was determined.