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Fig. 6. Histone H3 methylation monitored in isolated RGCs. (A) THY1 immunostaining of ganglion cells purified with anti-THY1 antibody. (B-E) Chromatin from purified RGCs was immunoprecipitated with an antibody directed against the dimethylated K4 of histone H3. Enrichments of ATH5 (B), ß3 (C), NeuroM (D) and NeuroD (E) promoter sequences are normalized relative to the value observed for E6 (B) or E18 (C-E) in the corresponding whole retina experiment.