Fig. 5. Deviation of neural crest derivatives to the glial lineage. Parasagittal sections of the head region of embryos 48 hours after electroporation of LSox5 showing the formation of the trigeminal (A,B), facial (C,D), ciliary (E,F), and superior (G,H) ganglia. Serial sections immunostained for LSox5 (green in A,C,E,G) or EGFP (green in B,D,F,H) show the distribution of transformed neural crest cells that maintain LSox5 overexpression. The exposure time was reduced in A,C,E,G to visualise only LSox5-overexpressing cells with signals above the normal level in glial cells. These cells are preferentially located at the proximal entrance of the corresponding cranial nerve (yellow arrowheads) or at the distal exit point (white arrowheads). Transformed cells are excluded from the proximal region of the trigeminal ganglion (broken line in A,B), where neural crest derived neurons should differentiate, and there is a consistent failure to colocalise with the neuronal marker (Islet, in red).