Fig. 5. Dsh, and Stbm localization in PCP mutants. Apical confocal sections with MF (anterior) at left edge and equator at bottom. MF is at the left margin of all panels, except in G, where it is indicated by red arrowheads. Mutant tissue is marked by absence of ß-gal staining or GFP (blue). White/yellow arrows indicate PCP protein localization in R3/R4 pairs (rows 3 or 4) and arrowheads mark clusters with R4-like enrichment (row 5 onwards). Localization of Dsh and Fz-GFP (red) and Strabismus (green) are shown. Clones of null alleles of the respective PCP genes (as indicated) are shown. (A) dgo^–: no differences in Dsh localization are detected between wild-type and mutant tissue. (B) pk^–, dgo^–: loss of apical localization of Dsh. (C) dgo^–: no difference in Stbm localization between wild-type and mutant tissue. (D) pk^–: apical localization of Stbm is observed (although it is slightly reduced). (E) pk^–, dgo^–: loss of apical Stbm localization. (F) fmi^–: loss in apical localization of Stbm. (G) fmi^–: loss of apical Fz-GFP localization is observed.