Fig. 4. wg and dachs are required for overgrowth in fat mutant discs. Discs stained for VG (green) and NUB (magenta); discs shown in A-F are at 36-48 hours of third instar, those in G-H are at 48-60 hours. Cells that express only NUB correspond to the distal half of the proximal wing (Fig. 1). (A) Wild-type. (B) fat⁸/fat^G-rv. (C) wg^spd-fg. (D) wg^spd-fg fat⁸/wg^spd-fg fat^G-rv. (E) dachs¹. (F) fat⁸ dachs¹. (G) fat⁸/fat^G-rv disc, the overgrowth of the proximal wing is even more pronounced at this age, and the wing becomes highly folded. (H) wg^spd-fg fat⁸/wg^spd-fg fat^G-rv. Wild type and fat⁸ dachs¹ are not shown at this age, as they begin to pupate. Scale bar in B: 80 µm for A-H.