Fig. 5. Expression of four-jointed and dachsous during wing development. Expression of VG (green), fj-lacZ (cyan), ds-lacZ (green), DS (green) and WG (red) are shown. (A) Early (0-12 hours of third instar) disc, stained for VG and fj-lacZ. (B) Early third instar disc, stained for ds-lacZ and WG. White bars identify WG expression in the proximal wing. Images to the right of the dashed line show different channels of vertical sections of the same disc. (C) Mid-late third instar disc (24-36 hours) stained for VG and fj-lacZ. Asterisks highlight proximal regions where VG expression remains elavated relative to fj. (D) Early third instar disc, stained for DS and WG. DS protein is predominantly apical (arrow). (E-G) Early (E), mid (F) and late (G) third instar discs, stained for WG and fj-lacZ.