Fig. 9. Dachsous influences WG expression. Discs stained for WG (red), Fat (magenta), MYC (green) or GFP (green). (A) Early third instar disc with ds^UA071 mutant clones, marked by absence of GFP. In some cases, WG is relatively decreased within clones (asterisk), and relatively increased in flanking wild-type cells (arrows). (B) Late third instar disc with ds^UA071 mutant clones, marked by the absence of MYC. WG expression is increased within a clone (arrow). (C) Early to mid-third instar disc with clone overexpressing DS, marked by GFP. WG appears elevated in cells at the edge of the clone (arrows), and slightly decreased in more internal cells. (D) Mid-third instar disc with clone overexpressing DS, marked by GFP. Ectopic expression of WG is detectable outside the clone (arrows). (E) Clone overexpressing DS and FJ, marked by GFP. Arrows point to examples of ectopic WG. Asterisk indicates a region where WG expression is out of the plane of focus. (F) Early-mid third instar with clones overexpressing DS, stained for Fat. Image is a composite of projections through different focal planes. Fat appears to accumulate at the clone border (arrow), and to be depleted from neighboring cells.