Fig. 5. When overexpressed, vhnf1 requires val for its ability to repress both hoxb1a and ephrin-B2a in r4. Overexpression of vhnf1 by mRNA injection (25 pg) represses hoxb1a (blue) in r4 of wild-type (B) but not val mutant embryos (D). Similarly, injection of vhnf1 mRNA represses ephrin-B2a in r4 of wild-type (F) but not val mutant embryos (H). Dorsal views of 11.5-13 hpf embryos, anterior to the left, with krox20 in red.