Fig. 2. Pole cells in N14^m– embryos die after exiting the midgut. Pole cells in N14^m– embryos were labeled with a photoactivatable lineage tracer, caged fluorescein (green), and an antibody against Vasa (red). Anterior is towards the left and dorsal is upwards. (A-C) At stage10, uncaged-fluorescein/Vasa double-positive pole cells were observed on the surface of the midgut. (D-F) From stage 11 onwards, the fluorescein-marked cells disappeared. The remaining fluorescein-positive cells were left on the dorsal side of the midgut surface. (G-I) At stage 12, few fluorescein-marked cells were observed. Scale bar: 40 µm.