Fig. 6. N14 is an allele of wun2. (A) Gene organization of the N14 locus. The N14 mutant phenotype was not complemented by Df(2R)w45-19g or Df(2R)w73-1, but was by Df(2R)Np1. These deficiencies defined the N14 locus within 100 kb of a genomic region that contains nine identified and predicted genes. A series of transgenic constructs that was used to identify the responsible gene for the N14 mutant phenotype is shown below. Only the constructs in which wun2 was intact (white boxes), rescued the N14 mutant phenotype. (B) An 8 kb HincII fragment (Pwun2-8k) rescued the N14 mutant phenotype, but the Pwun2-8k fragment, which had a partial deletion in the wun2 RNA-coding region, did not. The N14 chromosome had a nonsense mutation at the 111th Trp codon of the wun2-coding region. Mobilization of a P-element insertion, EP2650, generated a wun2 deletion (wun2). Embryos from N14/wun2 females showed the N14 mutant phenotype, confirming that wun2 is the responsible gene for the N14 mutation (Table 1).