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Fig. 7. Distribution of wun2 RNA. Wild-type embryos (A-C) and embryos from wun2{Delta}/Df(2R)w45-19g females (wun2{Delta} embryos; D) were stained for wun2 RNA. (A) wun2 RNA was detected throughout the stage-2 embryo. (B,B') At stage 4, wun2 RNA remained in pole cells, while the signal in somatic cells disappeared. (B') Highlighting of the posterior pole of the embryo shown in B. (C) At stage 5, wun2 RNA was detected in pole cells and in a posterior stripe. (D) Stage 5 wun2{Delta} embryo showing the absence of wun2 RNA in pole cells but its presence in a posterior stripe.