Fig. 8. Pole cell-specific expression of wun2 rescues the maternal wun2 mutant phenotype. Embryos were stained for Vasa to visualize pole cells. (A-C) Embryos from a cross of wun2^N14/Df(2R)w45-19g; nanos-Gal4-VP16/+ females with Oregon-R males. (D-F) Embryos from a cross of wun2^N14/Df(2R)w45-19g; nanos-Gal4-VP16/+ females with EP2650 males. (G-I) Embryos from a cross of nanos-Gal4-VP16 females with EP2650 males. (D-F) Expression of wun2 in pole cells fully rescued the maternal wun2 mutant defect. These pole cells were moderately dispersed at the late stages of embryogenesis (F), as in embryos overexpressing wun2 in pole cells (I) (Starz-Gaiano et al., 2001). The maternal wun2 mutant phenotype was not rescued with nanos-Gal4-VP16 driver alone (A-C) or with no driver (data not shown).