Fig. 1. (A) Western transfer analysis of transformant clones generated using the gskA disruption construct. Axenically growing cells were harvested and GskA protein was analysed as described in methods. Protein loading and transfer onto the membrane was normalised by staining with Ponceau S. (B) GSK3 kinase activity measured in cell extracts from AX2 cells and gskA-null clones. Growing cells were harvested and GSK3 levels assayed as described (Ryves and Harwood, 1998). Each sample was assayed in triplicate and means and standard deviations are shown.