Fig. 5. (A) Analysis of the expression pattern of cell-type specific reporter constructs in a random integrant and an Ax2/gskA– strain. Stable transformants for each of the indicated lacZ reporter constructs were allowed to develop on water agar. The stained structure for each random integrant transformant clone is shown on the left and the equivalent gskA-null mutant structure is shown on the right. The developmental promoter, or promoter subfragment, that directs expression of the lacZ gene is indicated on the left. Slug stages are shown for strains transformed with all reporter constructs, except for strains transformed with psA-lacZ, where tipped aggregates are shown. All structures derive from pools of transformants. (B) Prespore vesicle staining in a random integrant and a gskA-null mutant. Slugs were stained for prespore vesicles as described in the Materials and methods section.