Fig. 2. Nuclei are mispositioned within Glued mutant photoreceptors. (A-K) All neuronal nuclei in a third instar eye–brain complex are labeled with anti-Elav (blue in C-H,K, white in B,J). Individual cells are labeled with GFP (green in C-H,K, white in A,I) using a heat-shock FLP Act-FRT-FRT-Gal4:UAS-GFP chromosome in a wild-type background (A-D) or a Glued¹ mutant animal (E-K). An individual wild-type photoreceptor is marked with GFP in A, and all neuronal nuclei labeled with anti-Elav in B. A and B are merged in C, with a higher magnification view of the marked photoreceptor containing a nucleus in the inset. (D) An individual photoreceptor in another sample is imaged from a side view, with apical at the top. In Glued¹ mutants, individual cells in the optic stalk in E (marked with arrowheads) are shown at higher magnification in F. Examples of trailing processes are marked with arrows in F. Cells in F marked with both an arrowhead and a letter are shown at right, with their corresponding letter, at higher magnification. An individual labeled cell in another animal is shown in I, with trailing process indicated by the arrow. I and J are merged in K.