Fig. 5. Glued nuclear mispositioning is enhanced by dynein intermediate chain reduction. Third instar eye discs were stained with anti-Elav. (A,B) Apical surface of eye disc. Glued¹ mutants show small areas devoid of apical photoreceptor nuclei (A), while dic²/+; Glued¹/+ animals (B) have much larger areas devoid of nuclei (arrowheads). (C,D) Basal surface of eye disc and optic stalk. The greater absence of photoreceptor neuron nuclei in apical regions of the eye disc in dic²/+; Glued¹/+ animals is not simply due to an absence of photoreceptor neurons, as large numbers of photoreceptor nuclei are clustered at the base of the optic stalk in both Glued¹ (C) and dic²/+; Glued¹/+ animals (D).