Fig. 7. Glued function is required to position Bolwig organ nuclei, where it is antagonized by kinesin heavy chain. Wild-type second instar eye-antennal discs stained with anti-Elav show no neuronal nuclei in the eye-antennal disc along the path of the Bolwig nerve (A), while animals expressing dominant-negative Glued in postmitotic Bolwig photoreceptor neurons, using either the Elav promoter (B) or Glass_38-1 (C), have neuronal nuclei (arrowheads in B,C) along the path of the Bolwig nerve (in C, anti-Chaoptin in red, anti-Elav in blue, arrow indicates Bolwig nerve). Misplaced Bolwig nuclei in animals that express dominant-negative Glued under Glass_38-1 control were quantified in D. The number of misplaced Bolwig neuron nuclei was reduced when animals were heterozygous for loss-of-function mutations in khc. ^*P<0.05.