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Fig. 5. Physical and functional interactions of Frodo and TCF3. (A) Dose-dependent effects of Frodo and Dapper RNA on the activation of pSiaLuc reporter by Dsh. (B) Levels of non-phosphorylated ß-catenin are not changed in stage 10 embryos injected with FrdMO and DprMO in all blastomeres at the eight-cell stage. (C) Frodo constructs used for transfections and microinjections. (D) The association of GST-TCF3 with HA-Frodo and HA-Frd337, but not HA-Frd186, in COS7 cells, revealed in a GST pull-down assay. (E) TCF3 associates with Frodo in Xenopus gastrulae. Four- to eight-cell stage embryos were injected with 200 pg of HA-TCF3 RNA and 1 ng of Flag-Frd337 RNA. Protein complexes were immunoprecipitated with anti-Flag antibodies from gastrula stage lysates. (F) The activation of the pSiaLuc reporter by TCF3-VP16 is suppressed by HA-Frd337, but not by HA-Frd186.