Fig. 3. Pattern expression of integrins during joint formation. Forelimbs of E14 mice were fixed and tissue sections prepared as described in Materials and methods. Sections at the level of autopod were stained for immunofluorescence with polyclonal antibodies against the 5 integrin subunit. (A) Section of the forming fingers stained for 5 integrins. (B,C) Consecutive sections from a joint in the developing fingers showing immunofluorescence staining for 5 integrin (B) and in-situ hybridization for Wnt14 (C). Serial sections of the ulna stained for 5 integrin (D), type X collagen (E) and IHH (F).