Fig. 1. Expression of neuropeptide mRNAs during embryonic development. (A) CHH, (B) MIH, (C) PDH, (D) RPCH; n=3-11 independent measurements. Error bars are ±1 s.e.m.; y-axes are logarithmic. ND, no specific product detected, – not sampled. (E) and (F) amplification and melt curves from standard copy-quantified PDH (10⁷) copies/capillary in comparison with cDNA and mRNA extracted from unfertilised eggs (two egg equivalents/capillary). For definition of developmental stages see Table 1.