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Fig. 5. Quantification of CHH and CCAP levels during embryonic development, measured by RIA. (A) Developmental profiles for both neurohormones, bars CCAP; line, CHH; n=5-13 for CCAP, 5-23 for CHH. (B) An HPLC-RIA analysis of CHH and CCAP immunoreactive fractions of a Sep-Pak purified sample of 5000 embryos. Filled bar, CCAP; grey bar, PO-CHH; open bar, XO-CHH (see Materials and methods for details of analysis).