Fig. 3. Gata2 expression in sympathetic ganglion primordia is blocked by the Hand2 inhibitor noggin. Chick embryos were treated with BSA (A,B) or noggin (C,D) at stage 14 and analysed at stage 19 for the expression of Sox10 (A,C) and Gata2 (B,D) in sympathetic ganglion primordia close to the dorsal aorta (da). Arrowheads point to primary sympathetic ganglia. (E) Sox10 and Gata2 expression is quantified by determining the area of Sox10- and Gata2-expressing cells. The mean±s.e.m. of at least three embryos is shown. ^** significantly different from BSA controls (P<0.01) (Student's t-test).